Molecular evidence for the presence of Leptospira borgpetersenii in synanthropic rodents in the Nautla region, Veracruz, Mexico

Rigoberto Gutiérrez-Molina, Anabel Cruz-Romero, Dora Romero-Salas, Gerardo Ballados-González, José Jiménez-Hernández, Nelly Ibarra-Priego, Ricardo Serna-Lagunes, Sokani Sánchez-Montes

Resumen


The genus Leptospira encompasses ten species of spirochetes capable of infecting mammals, particularly rodents. In Mexico, studies focused on the detection of Leptospira sp. in rodents are scarce, all of them restricted to three states of the Gulf of Mexico. For this reason, this work aimed to identify the diversity of Leptospira species associated with synanthropic rodents in Veracruz, a state where leptospirosis is endemic.  Rodents were sampled with Sherman traps placed in 10 Production Units across the Nautla region. Animals were euthanized and their kidneys removed. Subsequently, a 474-bp segment of the outer membrane protein LipL32, present in all pathogenic species, was amplified and sequenced. Sequences were compared vs. reference using the BLAST algorithm: a phylogenetic reconstruction was carried out using the Maximum Likelihood method.  In addition, the prevalence of infection in each Production Unit was estimated.  Twenty eight rodents of a single species (Mus musculus) were caught. Leptospira DNA was detected in 17 samples (62.9 %, CI95 % 42.3 to 80.6) from seven localities in the Nautla region. The sequences recovered exhibited 99-100% identity to each other and 99 % identity with Leptospira borgspetersenii sequences deposited in GenBank.  This study confirms the presence of L. borgspetersenii in rodents, particularly in M. musculus, in Mexico.  This study increases the inventory of pathogenic leptospires for the state of Veracruz to three species. 


Palabras clave


México; pathogens; small mammals; spirochetes.

Texto completo:

PDF (English) HTML (English)

Referencias


Alvarado-Esquivel, C., A. Cruz-Romero, D. Romero-Salas, A. Alvarado-Félix, M. Aguilar-Domínguez, J. L. Ochoa-Valencia, D. Alvarado-Esquivel, J. Hernández-Tinoco, J. A. Zamarripa-Barboza, and L. F. Sánchez-Anguiano. 2018. Apparently high Leptospira antibody seropositivity in donkeys for slaughter in three municipalities in Durango, Mexico. Journal of Veterinary Diagnostic Investigation 30:929–932.

Alvarez, M. A., L. P. Moles-Cervantes, D. G. Rosas, C. N. Vasquez, and F. S. García. 2005. Retrospective seroprevalence study of bovine leptospirosis in Mexico considering the ecological regions. Revista Cubana de Medicina Tropical 57:28–31.

Ballados-González, G. G., S. Sánchez-Montes, D. Romero-Salas, P. Colunga-Salas, R. Gutiérrez-Molina, L. León-Paniagua, I. Becker, M. L. Méndez-Ojeda, C. Barrientos-Salcedo, R. Serna-Lagunes, and A. Cruz-Romero. 2018. Detection of pathogenic Leptospira species associated with phyllostomid bats (Mammalia: Chiroptera) from Veracruz, Mexico. Transboundary and Emerging Diseases 65:773–781.

Bourhy, P., L. Collet, S. Brisse, and M. Picardeau. 2014. Leptospira mayottensis sp. nov., a pathogenic species of genus Leptospira isolated from humans. International Journal of Systematic and Evolutionary Microbiology 64:4061–4067.

Colombo, V. C., I. Gamietea, S. G. Loffler, B. F. Brihuega, and P. M. Beldomenico. 2018. New host species for Leptospira borgpetersenii and Leptospira interrogans serovar Copenhageni. Veterinary Microbiology 215:90–92.

Cruz-Romero, A., C. Alvarado-Esquivel, D. Romero-Salas, A. O. Alvarado-Félix, S. Sánchez-Montes, J. Hernández-Tinoco, and L. F. Sánchez-Anguiano. 2018. Seroepidemiology of Leptospira infection in backyard pigs in Durango State, Mexico. European Journal of Microbiology and Immunology 8:87–90.

da Silva, E. F., S. R. Félix, G. M. Cerqueira, M. Q. Fagundes, A. C. Neto, A. A. Grassmann, M. G. Amaral, T. Gallina, and O. A. Dellagostin. 2010. Preliminary characterization of Mus musculus-derived pathogenic strains of Leptospira borgpetersenii serogroup Ballum in a hamster model. American Journal of Tropical Medicine and Hygiene 83:336–337.

Espinosa-Martínez, D. V., D. S. Sánchez-Montes, L. León-Paniagua, C. A. Ríos-Muñoz, M. Berzunza-Cruz, and I. Becker. 2015. New wildlife hosts of Leptospira interrogans in Campeche, Mexico. Revista do Instituto de Medicina Tropical de São Paulo 57:181–183.

Levett, P. N. 2015. Systematics of leptospiraceae. Current Top Microbiology and Immunology 387:11–20.

Matsui, M., L. Roche, M. E. Soupé-Gilbert, M. Roudier, V. Moniquet, and C. Goarant. 2015. Experimental hamster infection with a strain of Leptospira borgpetersenii Ballum isolated from a reservoir mouse in New Caledonia. American Journal of Tropical Medicine and Hygiene 92:982–985.

Méndez, C., L. Benavides, A. Esquivel, A. Aldama, J. Torres, D. Gavaldón, P. Meléndez, and L. Moles. 2013. Pesquisa serológica de Leptospira en roedores silvestres, bovinos, equinos y caninos en el noreste de México. Revista de Salud Animal 35:25–32.

Moles-Cervantes, L. P., M. A. Cisneros-Puebla, D. G. Rosas, N. R. Serranía, and J. I. Torres-Barranca. 2002. Serological study of bovine leptospirosis in Mexico. Revista Cubana de Medicina Tropical 54:24–27.

Panti-May, J. A., R. R. C. De Andrade, Y. Gurubel-González, E. Palomo-Arjona, L. Sodá-Tamayo, J. Meza-Sulú, M. Ramírez-Sierra, E. Dumonteil, V. M. Vidal-Martínez, C. Machaín-Williams, D. DE Oliveira, M. G. Reis, M. A. Torres-Castro, M. R. Robles, S. F. Hernández-Betancourt, and F. Costa. 2017. A survey of zoonotic pathogens carried by house mouse and black rat populations in Yucatan, Mexico. Epidemiological Infections 145:2287–2295.

Sánchez-Montes, S., D. V. Espinosa-Martínez, C. A. Ríos-Muñoz, M. Berzunza-Cruz, and I. Becker. 2015. Leptospirosis in Mexico: Epidemiology and Potential Distribution of Human Cases. PLoS One 10:e0133720.

Tamura, K., G. Stecher, D. Peterson, A. Filipski, and S. Kumar. 2013. Mega6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30:2725–2729.

Thibeaux, R., D. Girault, E. Bierque, M. E. Soupé-Gilbert, A. Rettinger, A. Douyère, M. Meyer, G. Iraola, M. Picardeau, and C. Goarant. 2018. Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the Diagnosis. Frontiers in Microbiology 1:816.

Torres-Castro, M., L. Guillermo-Cordero, S. Hernández-Betancourt, E. Gutiérrez-Ruíz, P. Agudelo-Flórez, R. Peláez-Sánchez, J. F. Zavala-Castro, and F. I. Puerto. 2016. First histopathological study in kidneys of rodents naturally infected with Leptospira pathogenic species from Yucatan, Mexico. Asian Pacific Journal of Tropical Medicine 9:145–147.

Torres-Castro, M., B. Cruz-Camargo, R. Medina-Pinto, B. Reyes-Hernández, C. Moguel-Lehmer, R. Medina, J. Ortiz-Esquivel, W. Arcila-Fuentes, A. López-Ávila, H. No-Pech, A. Panti-May, I. Rodríguez-Vivas, and F. I. Puerto. 2018. Molecular detection of pathogenic Leptospira in synanthropic and wild rodents captured in Yucatán, México. Biomedica 38:51–58.

Vital-Brazil, J. M., I. T. Balassiano, F. S. Oliveira, A. D. Costa, L. Hillen, and M. M. Pereira. 2010. Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement. Memorias do Instituto Oswaldo Cruz. 105:353–355.

Yager, R. H., W. S. Gochenour, A. D. Alexander, and P. W. Wetmore. 1953. Natural occurrence of Leptospira ballum in rural house mice and in an opossum. Proceedings of the Society for Experimental Biology and Medicine 84:589–590.

Zárate-Martínez, J. P., J. V. Rosete-Fernández, A. Ríos-Utrera, F. T. Barradas-Piña, and S. Olazarán-Jenkins. 2015. Prevalencia de Leptospirosis y su relación con la tasa de gestación en bovinos de la zona centro de Veracruz. Nova Scientia 7:202–217.


Enlaces refback

  • No hay ningún enlace refback.


FACTOR DE IMPACTO 2020 (SCOPUS): 1.2


THERYA es publicada por la Asociación Mexicana de Mastozoología A. C.  Se distribuye bajo una Licencia de Creative Commons Atribución-NoComercial-SinDerivar 4.0 Internacional.

DERECHOS DE AUTOR Y DERECHOS CONEXOS, THERYA es una publicación digital cuatrimestral editada por la Asociación Mexicana de Mastozoología A. C.  Hacienda Vista Hermosa 107, Colonia Villa Quietud, Coyoacan 04960.  Distrito Federal, México.  Telefono (612) 123-8486, www.mastozoologiamexicana.org.  Editor responsable: Dr. Sergio Ticul Álvarez Castañeda (therya@cibnor.mx).  Reservas de Derechos al Uso Exclusivo No. 04-2009-112812171700-102, ISSN: 2007-3364 ambos otorgados por el Instituto Nacional de Derechos de Autor.  Responsable de la última actualización de este número, Unidad de informática de la Asociación Mexicana de Mastozoología A. C.  Dr. Sergio Ticul Álvarez Castañeda.  Instituto Politécnico Nacional 195.  La Paz, Baja California Sur, C. P. 23096.  Tel 612 123 8486.